en علوم پایه پزشکی Basic Medical Sciences تحقیقی Original article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> <span style="color:black">A </span>major<span style="color:black"> contributor to hospital-acquired infections, particularly in burn units, is <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>). Because this bacterium produces extended-spectrum &beta;-lactamases (ESBLs), antibiotic resistance is a significant treatment concern. In this work, <i>P. aeruginosa</i> isolates from burn victims in Tabriz were examined for antibiotic resistance patterns and the presence of the bla<i>_TEM</i> gene.</span><b><span style="font-size:9.0pt"></span></b><br> <b>Methods:</b> <span style="color:black">In this descriptive-cross-sectional study, 100 clinical isolates of <i>P. aeruginosa</i> were collected from patients hospitalized in the burn wards of Tabriz hospitals over a six-month period. Standard biochemical methods were used to identify microorganisms. Antibiotic resistance patterns were assessed by the disk diffusion technique according to clinical and laboratory standards institute protocols. Additionally, the presence of the bla<i>_TEM</i>gene was investigated by polymerase chain reaction, and ESBL production was confirmed by the combined disk test.</span><b><span style="font-size:9.0pt"></span></b><br> <b>Results:</b> The highest resistance rates were observed for levofloxacin (97%) and meropenem (92%), while the lowest was for ceftazidime (69%). Furthermore, 58% (58/100) of the isolates were ESBL-positive, half of which (50%, 29/58) carried the bla<i>_TEM</i> gene.<br> <b>Conclusion</b>: The results<span style="color:black"> of this study indicated that <i>P. aeruginosa</i> strains in burn units of Tabriz hospitals exhibited high antibiotic resistance. Half of ESBL-positive isolates carried the bla<i>_TEM</i> gene, highlighting the need for continuous monitoring of antibiotic resistance patterns and prudent use of antibiotics.</span><br> <span style="font-size:9.0pt"></span></span></span></div> Pseudomonas aeruginosa, Drug resistance, Beta-Lactamases, Burns 15 18 http://goums.ac.ir/jorjanijournal/browse.php?a_code=A-10-961-2&slc_lang=en&sid=1 Aboalfazl Jafari-Sales a.jafari_1392@yahoo.com 100319475328460010549 100319475328460010549 No Department of Microbiology, Kaz.C., Islamic Azad University, Kazerun, Iran; Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran Zahra Ghahremani zghahremani1380@gmail.com 100319475328460010550 0009-0002-9744-3707 No Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran Aylin Golestani Ayliin.golestanii@gmail.com 100319475328460010551 0009-0008-0886-9874 No Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran Mohadeseh Ghorbanpour Koulani Jadid mohadesehghorbanpour1383@gmail.com 100319475328460010552 0009-0005-0532-1801 No Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran Kosar Hosseini-Karkaj kosar22hosseini@gmail.com 100319475328460010553 0009-0001-1120-9665 No Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran Kosar Soleymanpour kosarsoleymanpoor@gmail.com 100319475328460010554 0009-0002-2559-4645 No Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran Mohammad Mahdi Salek Faramarzi faramarzi.madi@gmail.com 100319475328460010555 0009-0005-3002-2536 No Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran Mehrdad Pashazadeh mehrdadpashazadeh85@gmail.com 100319475328460010556 0009-0005-0694-4592 Yes Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran