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Showing 4 results for Gharehkhani
Mohammad Shokrzadeh , Elahe Gharehkhani , Mahboube Rahmati Kukandeh, Mahsa Hosseini ,
Volume 27, Issue 2 (7-2025)
Abstract
Background and Objective: Prostate cancer is one of the most common malignancies worldwide. Docetaxel (DTX) is proposed as a well-known compound for prostate tumor chemotherapy, and its function is based on inhibiting microtubule depolymerization, disrupting microtubule balance, and consequently delaying cell cycle progression. Complications of DTX include hypersensitivity reactions, red blood cell aggregation, neutropenia, neurological problems, such as paralysis, fluid retention, bronchospasm, refractory hypotension, ADRS, respiratory impairment, cardiac dysfunction, ventricular tachycardia, cystoid macular edema, optic nerve damage, conjunctivitis, and keratopathy. This study aimed to determine the effect of curcumin on DTX-induced apoptosis in the DU145 (prostate) cell line using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Methods: This descriptive analytical study was conducted on the DU145 (prostate) cell line, purchased from the National Genetic Resources Cell Bank, at the Cell Culture Laboratory of the F aculty of Pharmacy, Mazandaran University of Medical Sciences. Cells were passaged for exposure to the desired drugs. Groups included curcumin at concentrations of 2, 4, 6, 8, and 10 µg/mL and DTX at a concentration of 4.46 µg/mL. Cells were incubated in triplicate for 24 hours. For the MTT assay, the culture rate was 104 cells per well. Apoptosis testing was designed for four groups (DTX at a concentration of 4.46 µM, curcumin at a concentration of 2 µM combined with DTX at an optimal concentration, curcumin at a concentration of 10 µM combined with DTX at an optimal concentration, and curcumin at a concentration of 10 µM alone), with the culture rate of 5×105 cells per well in 6-well plates. After cell exposure, MTT and apoptosis determination assays were performed.
Results: DTX reduced the viability of DU145 (prostate) cells by approximately 50% (P<0.05). Groups treated with curcumin combined with DTX showed a dose-dependent decrease in cytotoxicity and an increase in the viability of DU145 (prostate) cells (P<0.05). Additionally, curcumin was able to reduce apoptosis in DU145 (prostate) cells by 90%.
Conclusion: Curcumin increases cell viability and reduces apoptosis in DU145 (prostate) cells.
Elahe Gharehkhani , Marzieh Megharad, Mahboube Rahmati Kukandeh , Mohammad Shokrzadeh ,
Volume 27, Issue 4 (12-2025)
Abstract
Background and Objective: Due to high metabolic activity and rich blood supply, the kidneys are exposed to high levels of reactive oxygen species (ROS) under pathological conditions, making them highly vulnerable to oxidative stress. Nephrotoxic agents, such as cisplatin, aminoglycosides, and radiocontrast agents induce the production of ROS in renal tubular cells, leading to lipid peroxidation, protein oxidation, and mitochondrial dysfunction. This study was conducted to determine the protective effect of L-arginine against amikacin-induced nephrotoxicity in normal African green monkey kidney epithelial cells (Vero) by evaluating oxidative stress parameters.
Methods: This descriptive-analytical in vitro study was conducted on Vero cell lines purchased from the National Genetic Resources Cell Bank. For all assays, the amount of cultured calls was 105. Prior to the induction of nephrotoxicity with amikacin (653.2 µg/mL), the cells were pre-treated for 24 hours with various concentrations of L-arginine (108, 216, 430, and 860 µM). Subsequently, to evaluate the effect of L-arginine on oxidative stress status, the variables of malondialdehyde (MDA), cell viability, and ROS were measured.
Results: In the assays for ROS levels and cell viability, all tested concentrations of L-arginine (108, 216, 430, and 860 µM) resulted in a significant reduction in ROS levels (30±1.5, 28±1.4, 25±1.2, and 21±1.0, respectively) and a significant increase in cell viability (55±5.2, 64±3.8, 72±2.9, and 84±4.7, respectively) (P<0.05). Regarding measurement tests of lipid peroxidation, L-arginine at 108 µM did not significantly reduce MDA levels; however, other concentrations (216, 430, and 860 µM) significantly decreased MDA levels to 0.80±0.02, 0.74±0.03, and 0.66±0.01, respectively (P<0.05).
Conclusion: The findings of this study demonstrate the ability of L-arginine to improve kidney cell viability parameters and increase glutathione (GSH) levels at all tested concentrations (108, 216, 430, and 860 µM). Furthermore, L-arginine at concentrations of 216, 430, and 860 µM significantly reduced lipid peroxidation.
Elahe Gharehkhani , Sajedeh Zibayi, Mahboube Rahmati Kukandeh , Ramin Ataee , Mohammad Shokrzadeh ,
Volume 27, Issue 4 (12-2025)
Abstract
Background and Objective: 5-fluorouracil (5-FU) is a widely used chemotherapeutic agent for colorectal cancer treatment; however, its genotoxicity can lead to deoxyribonucleic acid (DNA) damage in healthy cells. Lycopene and Coenzyme Q10 are natural antioxidants capable of exerting protective effects against oxidative damage. This study was conducted to determine the protective effect of lycopene combined with Q10 against 5-FU-induced genotoxicity in colorectal cancer (SW480) and bone marrow mesenchymal stem cells (MSCs) cell lines using the Comet assay.
Methods: This descriptive-analytical study was conducted on SW480 and MSCs cell lines obtained from the Iranian Genetic Resources Cell Bank at the Cell Culture Laboratory of the Faculty of Pharmacy in 2023. The SW480 and MSCs cell lines were cultured at a density of 104 and exposed to a single dose of 5-FU (1 µM) along with various concentrations of lycopene and Q10 (0, 10, 20, and 30 µM). For each cell line, seven groups were defined: A control group (without treatment); a 5-FU group at optimum concentration (1 µM); groups receiving Q10 at 10, 20, and 30 µM plus lycopene at 10, 20, and 30 µM, respectively, comined with receiving 5-FU at optimum concentration (1 µM); a group receiving Q10 alone (30 µM); and a group receiving lycopene alone (30 µM). Cytotoxicity was evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, and DNA damage was assessed via the Comet assay.
Results: 5-FU caused a significant decrease in cell viability and a significant increase in DNA damage (P<0.05). Lycopene and Q10 alone did not exhibit significant cytotoxicity. The combination of lycopene and Q10 with 5-FU culminated in increased cell viability and decreased DNA damage compared to the group treated with 5-FU alone.
Conclusion: Lycopene and Q10 demonstrated significant protective effects against 5-FU-induced genotoxicity in both SW480 and MSCs cell lines.
Sarah Rayzan , Mehrasa Shademani , Elahe Gharehkhani , Mahboube Rahmati Kukandeh , Mohammad Shokrzadeh ,
Volume 28, Issue 1 (3-2026)
Abstract
Background and Objective: Amiodarone is an effective antiarrhythmic drug used for cardiac arrhythmias. Due to the presence of iodine in its structure, it tends to accumulate in lipophilic tissues, including the lungs, leading to lung injury and other major adverse effects. The present study aimed to determine the protective effects of vitamin E and the aqueous extract of Thymus vulgaris (T. vulgaris) leaves against amiodarone-induced cytotoxicity in non-cancerous human lung fibroblast (HLF) and human lung cancer (A549) cell lines.
Methods: This descriptive-analytical study was conducted on HLF and A549 cell lines obtained from the Pasteur Institute of Iran in the cell culture laboratory at the Faculty of Pharmacy, Mazandaran University of Medical Sciences. The grouping for non-cancerous HLF and A549 cell lines included a negative control group, a positive control group (amiodarone at its inhibitory concentration [IC50]), treatment groups receiving T. vulgaris and vitamin E at concentrations of 10, 20, and 50 μM, and single-treatment groups receiving T. vulgaris and vitamin E at 50 μM. All experiments were performed in triplicate with an initial cell density of 104 cells per well. To evaluate amiodarone-induced cytotoxicity (5.6 μM), methylthiazolyldiphenyl-tetrazolium bromide (MTT) (48 h), reactive oxygen species (ROS) (6 h), and lipid peroxidation (LPO) (48 h) assays were performed.
Results: The addition of amiodarone to both A549 and non-cancerous HLF cells culminated in a significant decrease in cell viability percentage in the MTT assay (at a concentration of 50 µM T. vulgaris and vitamin E in the normal cell line, and
20 µM T. vulgaris and vitamin E in the A549 cell line). Moreover, it led to a significant increase in ROS (at concentrations of 20 and 50 µM T. vulgaris and vitamin E in the normal cell line, and 20 µM T. vulgaris and vitamin E in the A549 cell line), as well as malondialdehyde (MDA) levels in the LPO assay (at concentrations of 20 and 50 µM T. vulgaris and vitamin E in the normal cell line, and 20 µM T. vulgaris and vitamin E in the A549 cell line) (P<0.05). Furthermore, adding a combination of vitamin E and extract of T. vulgaris leaves in amiodarone-intoxicated non-cancerous HLF cells led to a significant increase in cell viability against IC50 toxicity, alongside a significant reduction in ROS and MDA levels (P<0.05).
Conclusion: The addition of amiodarone to both A549 and non-cancerous HLF cell lines induces cytotoxicity in these cell lines. Concomitant treatment with vitamin E and extract of T. vulgaris leaves in amiodarone-treated cells culminates in a reduction in oxidative damage and an increase in cell viability in non-cancerous HLF cells.
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